Phospho-sting antibody

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August undefined, 2024

WebJan 29, 2015 · The p-S442 antibody was unable to detect a clear MAVS signal in whole-cell lysates before or after Sendai virus infection. However, IP with the p-S442 antibody … WebCell Signaling Technology's Phospho-STING (Ser366) (D8K6H) Rabbit mAb (Alexa Fluor ® 647 Conjugate) is a Rabbit monoclonal antibody. The Phospho-STING (Ser366) (D8K6H) Rabbit mAb (Alexa Fluor ® 647 Conjugate) Antibody was generated using stimulator of interferon response cGAMP interactor 1, and STING1 as the antigen. It reacts with …

(19781) Phospho-STING (Ser366) (D7C3S) Rabbit mAb

WebThe interaction between STING and NCOA4 exacerbates lethal sepsis by orchestrating ferroptosis and inflammatory responses in macrophages. In Cell Death & Disease on 28 July 2024 by Wu, J., Liu, Q., et al.. WebWestern Blot analysis of Phospho-TBK1 (Ser172) performed on various cell lines using a Phospho-TBK1 (Ser172) monoclonal antibody ( Product # MA5-35869) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was … reading chair with tablet arm

Phospho-STING (Ser366) (D8K6H) Rabbit mAb (Alexa Fluor ® 647 …

WebEach phospho-specific antibody has been tested in three different flow cytometry buffer systems: IC Fixation and Permeabilization , Foxp3/Transcription Factor Buffer, and IC … WebPhospho-STING (Ser366) (D7C3S) Rabbit mAb recognizes endogenous levels of STING protein only when phosphorylated at Ser366. Species Reactivity: Human Source / … WebHowever, detection of phosphorylated proteins in western blots can be challenging. At Bio-Rad, we have developed 10 tips to ensure robust data generation and cleaner blots. Topics include: Inhibition of protein degradation and conservation of post-translational modifications during sample preparation. Selection of phospho-specific antibodies. reading chair uk

STING-dependent sensing of self-DNA drives silica-induced lung ... - Nature

Pharmacological activation of STING blocks SARS-CoV-2 infection

WebAnti-STING Antibody Products. Listed below are anti-STING antibodies from multiple suppliers. STING is a reported alias name for the human gene TMEM173, or 'transmembrane protein 173'. The 379-amino acid protein is a member of the TMEM173 family. The cellular localization is predicted to be cytoplasmic, mitochondrial and membrane-associated. WebPhospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (Product # 44-680G) analysis of sorbitol-stimulated PC12 cells illustrate that only the phosphopeptide corresponding to ERK1/2 (pT185/pY187) blocks the western blot signal. The signal cannot be blocked by generic pT or pY peptide nor the non-phosphorylated ERK1/2 (T185/Y187) peptide. reading chair overhead lampWebPhospho-STING (Ser365) Antibody (PA5-117271) in WB Western blot analysis of extracts of THP-1 cells using Phospho-STING (Ser365) Polyclonal Antibody (Product # PA5-117271). … reading chair with book holder

"WebAnti-Phospho-STING-S365 Antibody (CABP1199) SKU: CABP1199 UPC: Product type: Antibody Antibody Type: Polyclonal Antibody Reactivity: Human Reactivity: Mouse Host Species: Rabbit Isotype: IgG Synonyms: ERIS Synonyms: MPYS Synonyms: Mita Synonyms: STING Synonyms: Tmem173 Synonyms: STING-beta Synonyms: 2610307O08Rik " - Phospho-sting antibody

Phospho-sting antibody

4 Human Monocyte STING Activation Flow Cytometry Panel

WebWestern blot analysis of extracts of THP-1 cells using Phospho-STING (Ser365) Polyclonal Antibody (Product # PA5-117271). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in … WebCell Signaling Technology's Phospho-STING (Ser366) (D8K6H) Rabbit mAb is a Rabbit monoclonal antibody. The Phospho-STING (Ser366) (D8K6H) Rabbit mAb Antibody was generated using stimulator of interferon response cGAMP interactor 1, and STING1 as the antigen. It reacts with Human. This antibody has been shown to work in applications such …

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WebDec 6, 2024 · c Immunoblots of STING/IRF3 axis in the lung of WT and STING −/− mice, including phospho-STING, STING, phospho-TBK1, TBK1, phospho-IRF3, and IRF3, with β-actin as a reference.

WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and … WebThe Phospho-STING (Ser366) assay measures STING when phosphorylated at Ser366. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The Phospho-STING (Ser366) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is …

WebPhospho-STING-Ser366 polyclonal antibody (Stimulator of interferon genes protein) binds to endogenous Stimulator of interferon genes protein only when phosphorylated at Ser366. Phosphorylation by TBK1 leads to activation and production of IFN-beta. Following cyclic nucleotide (c-di-GMP or cGAMP)-binding, activation and translocation from the ... WebSep 20, 2024 · The primary p-STING antibody (phospho-STING (Ser365) (D1C4T), Cell Signaling Technology) was prepared at a 1:400 concentration in Antibody Dilution Buffer (1x PBS, 1% bovine serum albumin, 0.3% ...

WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: …

WebPhospho-STING (Ser366) Antibody recognizes endogenous levels of STING protein only when phosphorylated at Ser366. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to … reading chair with shelvesWebFull length recombinant STING (TMEM173) protein produced in 293T cells. This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Affinity purified. Upon receipt, store undiluted between 2°C and 8°C. Each lot of this antibody is quality control tested by Western blotting. how to stretch shirts that are too smallWeb55. A method of treating, inhibiting, reducing, ameliorating, and/or preventing a cancer and or metastasis in a subject comprising administering to a subject an adoptive immunothe how to stretch shoes diyWebMay 28, 2024 · Cells were fixed and stained with antibodies against phospho-STING (p-STING) (H) or IRF3 (I). Images shown are representative of three independent experiments. ... anti-STING (Cell Signaling Technology, 13647S), anti-STING (Proteintech, 19851-1-AP), anti–phospho-STING (Ser 366) (Cell Signaling Technology, 50907S), anti-TBK1 (Cell … how to stretch shirts outWebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression. reading chair under 50WebRabbit Phospho-STING-S365 Rabbit pAb (AP1199), validaed in WB and tested in Human,, Mouse. 100% Guaranteed. ABclonal provides trial size antibody samples for target detection. ... Western blot analysis of extracts of THP-1 cells, using Phospho-STING-S365 antibody (AP1199) at 1:1000 dilution.THP-1 cells were treated by PMA (80 nM) at 37℃ for … how to stretch shoeWebNational Center for Biotechnology Information how to stretch shoes overnight