WebThe injection volumes for all samples was 4 µL and runs using a gradient of mobile phase A (water and 0.1% formic acid) and B (80% acetonitrile with 0.1% formic acid) at 0.3 µL/min, … WebJan 27, 2024 · Here, lysates were added to Sera-Mag Beads (Thermo Scientific, #4515-2105-050250, 6515-2105-050250) in 10 μl 15% formic acid and ... pH 8.4 in the presence of 1.25 mM TCEP and 5 mM chloroacetamide (Sigma-Aldrich, #C0267 ... Proteins were precipitated with 12% trichloroacetic acid and analyzed by SDS–PAGE and ...
Cellular proteomic profiling using proximity labelling by TurboID …
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Formic acid 56302 Honeywell Research Chemicals
WebApr 13, 2024 · Sigma-Aldrich . H6501 . Sodium butyrate . Sigma-Aldrich . ... SDS-PAGE samples were prepare d by mixing the supernatants and 4x Laem mli . ... (2 – 30% in 0.1% formic acid), coupled to a ... WebMature osteoclasts were characterized by staining for TRAP activity using an Acid Phosphatase Leukocyte Kit (Sigma-Aldrich), and TRAP-positive ... sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting ... Mobile Phase A is water in 0.1% formic acid; mobile phase B is 95% acetonitrile, 5% water, and ... WebThe autosampler temperature and the column temperature were kept at 6 and 40 °C, respectively. Mobile phase A was a mixture of water (98%) and acetonitrile (2%), and mobile phase B was acetonitrile, both containing 0.1% formic acid (v/v). A gradient of 8–48% of B/min in 10 min was used with a flow rate of 0.3 mL/min. inconsistency\\u0027s z9